The excised DNA fragments of the TCR that are not destined to be incorporated into a recombinant receptor gene are also ligated at their ends, forming a variety of circular DNA by-products called T-cell receptor excision circles (TRECs).
TREC test for SCID : On January 1, 2008, Wisconsin (USA) became the first state in the world to screen all newborns for SCID through a method based on measurement of T cell receptor excision circles (TRECs) by polymerase chain reaction (PCR), using DNA extracted from newborn dried blood spots (Guthrie cards).
TRECs were initially used to monitor generation of new T cells in HIV-infected individuals receiving effective anti retroviral treatment.
Amplify and quantify TREC DNA
Amplify and quantify reference gene DNA
Plasmids are used as standards
TaqMan Probe
- sequence specific
- fluorescent signal
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